Journal of Taiwan Agricultural Research

Author:Uei-Chern Chen, Yih-Juh Shiau, Hsin-Sheng Tsay and Chi-Ni Hsia*

Abstract:

    In vitro shoot multiplication of Salvia miltiorriza Bunge using nodal segment explants has been investigated in this study. Aseptic shoots were established on a full-strength MS medium containing 1 mg/l benzyladenine (BA) and 0.1 mg/l α-naphthaleneacetic acid (NAA) for shoot multiplication. In the cytokinin for shoot multiplication experiment, among the concentrations of BA (0.05, 0.1 mg/l) and kinetin (0.25, 0.5 mg/l) tested, the highest shoot multiplication (6.5 shoots per explant) was achieved in the medium containing 0.25 mg/l kinetin after 6 weeks of culture. Nevertheless, hyperhydric shoots were found for all cytokinin treatments. In order to improve the hyperhydricity disorder for in vitro shoot proliferation of S. mitrorriza, a medium with half-strength MS basal salts and lower concentration of kinetin was used for subsequent experiment. The highest normal shoots (3.0 per explant) were obtained from the 0.25 mg/l kinetin treatment along with a 46.6% hyperhydricity. Using the dispense paper instead of aluminum foil as container closure improved ventilation and reduced hyperhydricity in shoot cultures. Both the highest normal shoots (4.8 per explant) and the longest shoot length (4.78 cm) were obtained in the treatment using aluminum foil as container closure for the first 2 weeks of culture, then exchanging with dispense paper for the rest 4 weeks of culture. In conclusion, an in vitro shoot proliferation system for S. miltiorriza was established by culturing shoots in the medium containing half-strength MS basal salts and 0.2 mg/l kinetin. However, using ventilating dispense paper as container closure for certain culturing period was beneficial to overcome the hyperhydric disorder in shoot cultures.

Key words:Salvia miltiorrhiza, Medicinal herbs, Micropropagation, Hyperhydricity, Ventilating container closure

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