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A Fast Method for Screening Amylose Mutant Rice
The three steps for growing embryo-containing half rice grain: (A) germination in culture tube, (B)transplanting, and (C)pot cultivation. The medium used for germination step was sterilized agar or sterilized soil then the seedlings were transplated to the pot tilled with paddy soil.
The three steps for growing embryo-containing half rice grain: (A) germination in culture tube, (B)transplanting, and (C)pot cultivation. The medium used for germination step was sterilized agar or sterilized soil then the seedlings were transplated to the pot tilled with paddy soil.

Author:Toong-Long Jeng, Su-Yue Lin, Tong-Hai Tseng and Chang-Sheng Wang*

Abstract:

    Rice grains with various amylose contents from low (7%)、 high (30%) and varied (non-purified) amylose mutants, as well as from their original variety, TNG67 (20%), were applied in this study. A method with less labor cost for amylose analysis on a single grain and half grain is developed in this study. The distribution of amylose in grains of all spikelets on a panicle could be detected and planted to the next generation for further analysis, and a non-purified segregating mutant, SA0404, was quickly screened and purified by this method. Besides, our investigations found that there is no difference in amylose content between distal and proximal part of a single rice grain. In this report, a fast and reliable method for analyzing amylose content and plant growing system on a single rice grain was developed that will benefit to improve rice quality related amylose content.

Key words:Rice, Amylose, Mutant

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Journal of
Taiwan Agricultural Research
Vol. 73, No.1 (2024) ISSN: 2790-086X Vol. 73, No.1 (2024)
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