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Author:Dong-Hong Wu and Kae-Kang Hwu*
Abstract:
Objective of this study is to determine the genetic consistency of seeds produced under three-step propagation system. Twenty-three simple sequence repeat (SSR) markers which have full discrimination power on 22 superior rice varieties produced in Taiwan during 2004 and 2005 were used in this study. Breeder’s seeds and foundation seeds of these 22 varieties were used to establish standard genetic profiles for each variety. The result shows that allelic composition of foundation seeds possessed single genotype and was identical with breeder’s seeds in most varieties. But allelic composition of TK11, TK16, KH139, KH145 and TNGS22 contained more than one genotype. Cluster analysis of 77 registered samples in 2005 first season and their predecessors, breeder’s seed, reveals high genetic consistency except TK9, TNG71 and TK16. In final, it is suggested to extend one more generation for plant selection until F5 gneration, in order to increase seed identical ratio from 87.5% to 93.5%.
Key words:Rice, Three-step propagation system, SSR, Japonica rice, Indica rice, Oryza sativa
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