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Studies on in vitro Shoot Multiplication of Native Medicinal Herbs - Bupleurum kaoi by Axillary Bud Culture
Plant material and establishment of shoot cultures of Bupleurum kaoi. Growth of new shoots in one-year-old Bupleurm kaoi plant maintained at Agricultural Research Insitute's farm in Taichung (a), Sprouted shoot in the axillary bud after two weeks (b) and proliferated shoots after six weeks (c0 of culture on half-strength MS basal medium supplemented with 1 mg/l BA and 0.1 mg/l NAA, Established shoot culture using axenic explant (in vitro grown shoot, 1.5com in length) on the same medium after 6 weeks of culture(d).
Plant material and establishment of shoot cultures of Bupleurum kaoi. Growth of new shoots in one-year-old Bupleurm kaoi plant maintained at Agricultural Research Insitute's farm in Taichung (a), Sprouted shoot in the axillary bud after two weeks (b) and proliferated shoots after six weeks (c0 of culture on half-strength MS basal medium supplemented with 1 mg/l BA and 0.1 mg/l NAA, Established shoot culture using axenic explant (in vitro grown shoot, 1.5com in length) on the same medium after 6 weeks of culture(d).

Author:Uei-Chin Chen, Mau-Shing Yeh and Hsin-Sheng Tsay*

Abstract:

    An efficient method for in vitro shoot multiplication of Bupleurum kaoi Liu, Chao et Chuang using the axillary bud explants has been developed. The axillary buds dissected from nodal segments of the field grown plants induced shoots after culturing for 4 weeks on half-strength MS medium supplemented with 1 mg/l benzyladenine (BA) in combination with 0.1 mg/l α-naphthaleneacetic acid (NAA). Among the various concentrations of BA (0 - 2 mg/l) or kinetin (0 - 0.8 mg/l) tested, maximum shoot multiplication (7.3 shoots per explant) was achieved in the in vitro grown shoots by culturing on half-strength MS medium supplemented with 0.25 mg/l BA for 8 weeks. Although BA was more effective than kinetin in shoot multiplication, BA stimulated hyperhydric shoots (10 - 33%) after 6 weeks of culture. Prolonged culture period from 6 weeks to 8 weeks resulted in higher number of hyperhydric shoots (16 - 47%). Half-strength of MS basal medium and 3% sucrose as carbohydrate source was found optimum for shoot multiplication. Pretreatment of the explants by culturing them on liquid half-strength MS basal medium containing 0.25 mg/l BA for 2-14 days did not support shoot multiplication.

Key words:Bupleurum kaoi, Medicinal plant, Shoot multiplication, Hyperhydricity

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Journal of
Taiwan Agricultural Research
Vol. 73, No.1 (2024) ISSN: 2790-086X Vol. 73, No.1 (2024)
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