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Evaluation and Genetic Relationship Analysis by RAPD on Oriental Plum Germplasm
Dendrogram illustrating enetic relation ship among 30 plum varieties, constructed by Jaccard's distance and UPGMA clustering method from 224 RAPD markers
Dendrogram illustrating enetic relation ship among 30 plum varieties, constructed by Jaccard's distance and UPGMA clustering method from 224 RAPD markers

Author:Ien-Chie Wen* and Yi-Ling Liu

Abstract:

    Taiwan local plums are adapted to subtropical climates with a low cu winter and become the important low chill plum genepool in the world. Varisties “Huang jou” and “Huang kan” have a distinct aroma, and “Hei tao”, “Hei tzyy”, and “Shui li” have sweet skin (non-astringent). All of them are valuable parents for low chill plum breeding. The genetic relationships of 30 plum cultivars were analyzed by using RAPD markers. A total of 320 primers were screened for their ability to produce strongly amplified products. Out of 30 primers amplified 224 DNA bands, 190 of which were polymorphic (84.8%). A dendrogram based on the UPGMA cluster analysis was constructed. All tested plum cultivars were divided into nine groups, including 4 groups of local varieties. Varisties “Tung shan sha lien”, “Sha lien” and “Tou pan” had 94% similarity indexes, indicating that they have the closest genetic relationship. Nevertheless, variety P. pissardi has the farest genetic relationship with other tested plum variety due to only 32% similarity indexes among them.

Key words:Prunus salicina, Germplasm evaluation, RAPD

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Journal of
Taiwan Agricultural Research
Vol. 72, No.3 (2023) ISSN: 2790-086X Vol. 72, No.3 (2023)
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