Journal of Taiwan Agricultural Research

Author:Ming-Jen Fan, Shu Chen* and L. M. Engle

Abstract:

    The objective of this study was to evaluate the genetic diversity of Capsicum core collection by RAPD. The core collection which contains 264 accessions of Capsicum spp. was constructed with 12 ratio scale traits. The cluster analysis based on RAPD from 16 random primers showed the accessions of core collection could be divided into 5 clusters as the mini distance (MD) was greater than 0.83. Most (92%) of the C. annuum were assembled in cluster 1, 92% of the C. baccatum were in cluster 4, 86% of the C. chinense were in cluster 2, 86% of the C. frutescens were in cluster 3, and all of the C. chacoense were in cluster 5. Cluster analysis revealed there are obvious molecular diversity among species within Capsicum. Therefore, it is recommend that the Capsicum core collection should cover the germplasm from different species as many as possible. For the accessions from the same species, there are still some scattered to the other clusters. The result showed certain of molecular diversity existed within species. Aimed at the viewpoint to conserve broad genetic diversity, these distinct germplasm should be included in core collection. The Shannon-Weaver diversity index of the cluster which primarily composed of C. annuum was 0.783, which revealed there are higher similarity within this cluster. The accessions within the cluster could be reduced by way of RAPD analysis. This study showed the RAPD molecular markers not only provided insights into the genetic structure, but also assisted the construction of core collection for Capsicum.

Key words:Capsicum, Core collection, RAPD, Genetic diversity

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