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Effect of Pollination Stage and Cold Storage of Capsule on the Seed Germination of Haemaria discolor (Ker) Lindl.
An ornamental and medicinal important terrestrial orchid-Haemaria discolor (Ker) Lindl. A. Plants and leaves of Haemaria discolor. Bar=2m, B. The inflorescence and flowers. Bar=2cm, C. The massula showing emergence of pollen tubes (arrows). Bar=41μm, D. Appearance of seed germination after 30 days cultured in vitro (arrows). Bar=1mm.
An ornamental and medicinal important terrestrial orchid-Haemaria discolor (Ker) Lindl. A. Plants and leaves of Haemaria discolor. Bar=2m, B. The inflorescence and flowers. Bar=2cm, C. The massula showing emergence of pollen tubes (arrows). Bar=41μm, D. Appearance of seed germination after 30 days cultured in vitro (arrows). Bar=1mm.

Author:Yih-Juh Shiau, Chi-Ni Hsia* and Hsin-Sheng Tsay

Abstract:

    Haemaria discolor (Ker) Lindl. is cultivated for the purpose of ornamental and medicinal in Taiwan. We have optimized a method for artificial self-pollination of H. discolor and for proloning capsules storage life by using cold treatment in this study. The highest fruit set (100%) and embryo germination (44.6-45.6%) were obtained when the pollinia and ovules from flower stage of 0-5 DAF (days after flowering) used for pollination. The germination rate of massules from 3-5 DAF stage were 98.4% (82.6 pollen tubes/massule) when culturing in the liquid medium containing 10% sucrose with 0.01% boric acid for l8hrs. Cold treatment on capsules at 5±1℃ with 100% relative humidity was found effective to prolong seed longevity to 28 days.

Key words:Haemaria discolor, Orchidaceae, Pollination, Seed germination

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Journal of
Taiwan Agricultural Research
Vol. 73, No.1 (2024) ISSN: 2790-086X Vol. 73, No.1 (2024)
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