Journal of Taiwan Agricultural Research

Author:Uei-Chern Chen, Chi-Ni Hsia, Mau-Shing Yeh and Hsin-Sheng Tsay*

Abstract:

    An efficient protocol for producing high quality rooted plantlets which are easy to acclimatize for field growth was established for micropropagation of Bupleurum kaoi Liu, Chao et Chuang. In this study, the medium containing half- and quarter- strength of MS basal salts (1/2MS and 1/4MS) and 3% (w/v) sucrose was found suitable for shoot survival and root formation of in vitro B. kaoi plantlets. A l00% root formation rate was obtained from in vitro shoots cultured on the medium containing half-strength MS salt, 0.5 mg/l indole-3-butyric acid (IBA) without basal callus further induced. An increased survival rate for in vitro plantlets acclimation by using ventilative container closure of dispense paper to exchange the normal used aluminum foil after 2-3 weeks culturing in a total 6 weeks incubation span was achieved. Leaves of in vitro rooted plantlets were partial trimmed (3-4 cm remnant in length) and transplanted into BioMix: vermiculite: perlite = 1: 1: 1 (v/v) mixed substrate inside a transparent plastic box with cover. Boxes were put into a growth chamber under 22℃ (14 h in light) / 18℃ (10 h in darkness) environmental condition for 4 weeks acclimation. The best survival rate was 92% after 4 weeks of acclimation from plantlets previously cultured on half-strength MS medium containing with 0.5 mg/l IBA and 0.1-0.2 mg/l α-naphthaleneacetic acid (NAA), in combination with changing aluminum foil with 3 layers of dispense paper as container closure after 2 weeks culturing. These results including in vitro treatment by modifying medium components and exchanging container closure for root formation and plantlets quality; ex vitro treatment by planting plantlets inside a transparent plastic box with cover for reducing transpiration would be useful for industrial production of in vitro Bupleurum kaoi plantlets.

Key words:Bupleurum kaoi, Medicinal plant, Rooting, Acclimation, Container closure

Download: PDF Links