Journal of Taiwan Agricultural Research

Author:Su-Chen Lin, Su-Yuang Lin and Chi-Guang Wu*

Abstract:

    Three different herbicides, i.e., Isopropylamine salt of N-phospho-nomethyl glycine (glyphosate), 1,1-Dimethyl- 4,4-bipyridylium dichloride (paraquat), and Bensulfuron- methyl benzoate(londax), were evaluated in vitro for their influences on the spore germination and hyphal growth of four vesicular-arbuscular mycorrhizal fungi, Gigaspora albida Schenck & Smith, Glomus mosseae (Nicol. & Gerd.) Gerdemann & Trappe, Scutellospora fulgida Koske & Walker, and Acaulospora tuberculata Janos & Trappe. The dosages of each herbicide used in the study were 4.1, 1.2, and 1g/L respectively. After surface sterilization, 50 spores per plate (9 cm in diameter) were pipetted on water agar amended with different concentration of herbicides. Water agar without herbicide amendment was treated as controls. The results indicated that glyphosate could completely inhibit spore germination of Gigaspora alibida, Glomus mosseae, Scutellospora fulgida, and Acaulospora tuberculata. Paraquat did show the same inhibition effect as glyphosate on those species, except Gi. albida, whose germination rate was 7.2% and mycelial growth was 7.8 mm after 18 days herbicide treatment, in contrast to the controls whose germination rate was 47.2% and hyphal length was 72 mm. Londax inhibited significantly the germination and hyphal growth of G. mosseae and A. tuberculata; however, with regard to the species of Gi. albida and S. fulgida, it showed different influence pattern. The herbicide did not reduce the germination rate significantly, but it restrained the hyphal growth notably. In general, Gi. albida showed more tolerance to the herbicides used in this study than other species.

Key words:Herbicide, Arbuscular mycorrhizal fungi, Spore

Download: PDF Links