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Author:Shu-Ru Yang*, Charng-Pei Li, Shu-Tu Wu and Fu-Sheng Thseng
Abstract:
In order to understand the seedling characters of different dwarf genes expressed under 5 and 7 cm seeding depth, Var. Shiokari and its seventeen dwarf near-isogenic lines were used for investigation. The results were that the emergence ability of d-1, d-2, d-18k and d-42t was significantly inhibited at 5cm seeding depth, but d-6 was enhanced, and no significant difference was found among the rest dwarf near-isogenic lines with Shiokari. All dwarf near-isogenic lines were emerged with the elongated of mesocotyle, however, coleoptile internode and incomplete leaf internode however d-1 was found emerged by its 1st leaf internode. At 7cm seeding depth, the emergence ability of sd-1, d-6, d-12, d-14 and d-52 were enhanced however d-1 was inhibited and no difference found among the rest dwarf near-isogenic lines with Shiokari. All dwarf near-isogenic lines were emerged by the elongation of incomplete internode and the 1st leaf internode however d-1 was found emerged by the 2nd leaf internode. Each dwarf genes had different effects along with various degrees on seedling characters at both seeding depth. Most seedlings of Shiokari and its dwarf near-isogenic lines emerged by the 1st leaf. It was found the emerged ratio of the 1st leaf was increase and which the seeding depth however the emergence of 2nd leaf was also observed on some seedings. Through principal components analysis, it was found that Shiokari and its most near-isogenic lines were distributed in 1st quadrant at 5cm seeding depth however the distribution in 4th quadrant was found while seeding depth at 7cm. It was observed that the distribution of dwarf near-isogenic lines were not scattered near Shiokari. Conclusion from principal components analysis and genotypic correlation, it was found that dwarf genes had pleiotropic effects on seedling growth under deep seeding culture.
Key words:Deep Seeding, Dwarf Near-isogenic Lines, Emergence Ability, Genotypic Correlation, Principal Components Analysis
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