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The Genetic Diversity Analysis of Rhododendron Native in Taiwan
RAPD dendrogram of 23accesions of Rhododendron native in Taiwan generated by 7 primers (OPE3, OPE7, OPE15, OPE19, OPE20, OPE3 and OPR16), and constructed by UPGMA cluster analysis wihch based on the Jaccard's correlation matrix. Sample no. 1, 5, 6, 15: R. simsii, 2:R. formosanum, 3:R. ellipticum, 7, 8, 9, 10, 11, 12:R. oldhami, 13:R. rubropilosum, 14: R.hyperthrum, 16:R.breviperulatum, 17,18, 19:R. kanehirai, 20:R. ovatum, 21:R. noriakianum, 22:r. mariesii, 23:R. sp.
RAPD dendrogram of 23accesions of Rhododendron native in Taiwan generated by 7 primers (OPE3, OPE7, OPE15, OPE19, OPE20, OPE3 and OPR16), and constructed by UPGMA cluster analysis wihch based on the Jaccard's correlation matrix. Sample no. 1, 5, 6, 15: R. simsii, 2:R. formosanum, 3:R. ellipticum, 7, 8, 9, 10, 11, 12:R. oldhami, 13:R. rubropilosum, 14: R.hyperthrum, 16:R.breviperulatum, 17,18, 19:R. kanehirai, 20:R. ovatum, 21:R. noriakianum, 22:r. mariesii, 23:R. sp.

Author:Ming-Jen Fan*, Jau-Yueh Wang, Shuen-Fang Lo and Yen-Hsueh Tseng

Abstract:

    A total of 23 accessions including 11 species of Rhododendron native in Taiwan which were collected from different areas, were evaluated by using randomly amplified polymorphic DNA (RAPD) marker. Objectives were to evaluate genetic distance within those 11 species. Screening from sixty primers and eighteen unique DNA fragments were derived from 7 polymorphic primers. They can be used to distinguish the 7 species from the total 11 species, such as R. oldhamii, R. rubropilosum, R. ovatum, R. hyperthrum, R. ellipticum, R. breviperulatum, and R. mariesii. A dendrogram generated by UPGMA (Unweighted Pair-Group Mean Arithmetic) cluster analysis is closed relationship with the taxonomic classification of “Flora of Taiwan”. Four sub-groups can be clustered by RAPD results. The results also show the varieties in the ‘R. oldhami’ were clustered into the same subgroup. In brief, that the RAPD markers is an effectively and reliably method for analysis of the genetic distance of Rhododendron native in Taiwan.

Key words:Rhododendron, RAPD, Cluster analysis, Genetic diversity

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Journal of
Taiwan Agricultural Research
Vol. 72, No.3 (2023) ISSN: 2790-086X Vol. 72, No.3 (2023)
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