Journal of Taiwan Agricultural Research

Author:Huey-Jiuan Huang, Shun-Fu Lin and Jaw-Shu Hsieh

Abstract:

In this study RAPD and SSR markers were used to analyze genomic context of interspecific hybrids (A. hypogaea L. × A. spegazzinii L.) of peanut. Frequencies and parental origins of DNA polymorphic bands were investigated for the interspecific hybrids (4x×2x). The results indicated that about 11 bands were PCR amplified from total genomic DNA of peanuts with intra-SSR primers of rice or corn, and about 7 bands were amplified with inter-SSR primers. The averaged polymorphic rates were 45.4% and 45.1% for a pair of intra-SSR primer and an inter-SSR primer, respectively. However, the averaged polymorphic rate for a RAPD primer was 41.3%. The percentages of a (pair of) primer with polymorphic bands between interspecific parents were 34.2, 36.0% and 33.6% for intra-SSR (rice/corn), inter-SSR, and RAPD primers, separately. About twice (66.8%) polymorphic bands were contributed from allotetraploid parent compared to diploid parent (33.2%) providing molecular evidence of genomic context of interspecific hybrids and inconsistence between genomic contribution and phenotypic variation. This information is valuable for genome and gene expression studies as well as the utilization of interspecific hybridization in plant breeding.

Key words:Peanut (Arachis hypogaea), Interspecific hybrids, Molecular marker

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