Journal of Taiwan Agricultural Research

Author:Su-Chen Lin, Su-Yuang Lin and Chi-Guang Wu

Abstract:

The main purpose for this experiment was to evaluate the effect of VA mycorrhizal fungi (VA), phosphatesolubilizing (PS), and free-living nitrogen-fixing (NF) bacteria on the growth of Eustoma grandiflorum. Plants with 4 true-leaves were used for inoculation. Bulbs of calla lily were grown in the vermiculite-perlite-peat medium (6 : 3 : 1, v/v/v) with day/night temperatures as 23/18℃ and were fertilized with modified Hoagland’s solution weekly. The growth effect of the microbes to calla lily was investigated and measured 116 days after inoculation. Treatments inoculated with either VA mycorrhizal fungus (VA) and free-living nitrogen- fixing bacteria (NF) have significantly higher dry weight of shoot (17% and 52%, respectively) and dry weight of root (56% and 50%, respectively) than those of uninoculated treatments (control). The plant growth of the treatment inoculated with phosphate-solubilizing bacteria (PS) was not significantly different from that of control. Any combination of VA+PS, VA+NF, or VA+PS+NF could promote 30%, 42%, and 39% dry weight of shoot, and 53%, 53% and 56% dry weight of root, respectively. Treatment inoculated with VA+NF and VA+NF+PS produced 2.4 and 3.2 flowers per plant, respectively, more than that of controls. Furthermore, these two treatments flowered 14 days earlier. Adding PS or NF to the VA inoculation, or mixing the three inoculants together caused slower in colonization rate. However, the shoot dry weight of each treatment is heavier than that of single VA inoculation. There is no promoting effect on phosphate uptake by plants among those different microbial inoculations.

Key words:Eustoma grandiflorum, Arbuscular mycorrhizal fungi, Free-living nitrogen-fixing bacteria, Phosphate-solubilizing bacteria, Phosphorus

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