Journal of Taiwan Agricultural Research

Author:Yih-Juh Shiau, Chung-Der Tai, Chung-Chuan Chen and Hsin-Sheng Tsay

Abstract:

Bei-mu was first recorded in Shen-Nung-Pen-Ts’ao-Ching under the middle herb catogory and was recorded in successive Pen-ts’aos of the descending dynasties. It has been used as anti tissue and expectorant for the care of coughing and the prevention of phlegm formation. As the amount of bulb collected from naturally-grown and traditionally-cultured plants is far from enough for medicinal demand, tissue culture method of mass propagation was therefore studied. For the comparison on the efficiency of new bulbscale production among various explants cultured on solid medium containing MS basic salts and 0.5 mg/l BA concentration for 60 days, bulbscaie explant showed the highest percentage (54.7%) followed by stem base protocorm-like-body (PLB), and leaf blade. On the contrary, the order of browning percentage during the culturing period was reversed for the four explants. The stem base explant could produce the highest number of bulbscale (5.6 per culture). The induction percentages of embryogenic callus were higher for PLB and stem base (36.1% & 30.6%, respectively). Addition of 0.5 mg/l BA and 0.25-4 mg/l NAA to the MS medium resulted in increased efficiency of bulbscale formation, e.g., from 68-72% for stem base explant and 62-78% for bulbscale explant. NAA in the concentration range of 4 mg/l could also promote the rooting ability and embryogenic callus formation of the explants.

Key words:Fritillaria hapehensis Hsiao et K.C.Hsia, Protocorm like body, Tissue culture, Mass propagation

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