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Induction of Morphological Variants from Stem Node Explants of Philodendron after Treatment with γ -rays
Multiple shoot formation in stem node explants of four cultivars of Philodendron when cultured on MS medium with 1.0 mg/l BA and 0.2 mg/l NAA. (A) Philodendron cv. 'Tuxla', (B) Philodendron cv. 'Imperial Green', (C) Philodendron cv. 'Pluto', (D) Philodendron cv. 'Red Princess'.
Multiple shoot formation in stem node explants of four cultivars of Philodendron when cultured on MS medium with 1.0 mg/l BA and 0.2 mg/l NAA. (A) Philodendron cv. 'Tuxla', (B) Philodendron cv. 'Imperial Green', (C) Philodendron cv. 'Pluto', (D) Philodendron cv. 'Red Princess'.

Author:Uei-Chin Chen, Yih-Juh Shiau and Hsin-Sheng Tsay

Abstract:

    Stem node explants derived from in vitro propagated shoots of four cultivars of Philodendron viz. ‘Tuxla’, ‘Imperial Green’, ‘Red Princess’, and ‘Pluto’ were irradiated with γ -rays (20 and 40 Gy) to induce variation. The explants were pre-cultured in liquid Murashige and Skoog’s (MS) medium supplemented with 2.0 mg/l N6-benzyladenine (BA) or 0.5 mg/l thidiazuron (TDZ) before irradiation. After irradiation, the explants were cultured on MS medium supplemented with 1.0 mg/l BA + 0.2 mg/l (- naphthaleneacetic acid (NAA) for production of multiple shoots. Morphological variants were observed only in cv. ‘Red Princess’. Other three cultivars, though could be successfully propagated in vitro, failed to show any morphological variation after treatment with γ -rays.

Key words:γ -rays, Philodendron, Tissue culture, Morphological variation

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Journal of
Taiwan Agricultural Research
Vol. 73, No.1 (2024) ISSN: 2790-086X Vol. 73, No.1 (2024)
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