Journal of Taiwan Agricultural Research

Author:Ying-Huey Cheng*, Chin-Chih Chen, Jye-Yann Liao, Ting-Chin Deng, and Chin-An Chang

Abstract:

    Lisianthus plants exhibited stunting and yellow spots were collected from Chiayi county in 2004.  Virus isolates were obtained through 3 successive single lesion passages on Chenopodium quinoa and then propagated on Nicotiana benthamiana.  The virus isolate caused mild mottle symptoms on bell pepper plants and faint yellow spots on lisianthus leaves.  Virus particles were purified from infected N. benthamiana by polyethylene glycol precipitation and then followed by Cs₂SO₄ isopycnic centrifugation.  Relative molecular weight of the coat protein of the virus was 31 kDa when analysis by SDS-PAGE.  Antiserum has been produced by immunizing rabbit with virus particles.  Indirect ELISA and Western blotting analyses indicated that the lisianthus virus was serologically related to Chilli veinal mottle virus but not to Potato virus Y and Turnip mosaic virus.  Using the potyvirus degenerate primers, a 1.4 kb DNA fragment was amplified by reverse transcription-polymerase chain reaction (RT-PCR).  Sequence data of the amplified fragment revealed that the causal virus was an isolate of  Pepper veinal mottle virus as it shared 99.3% identities to the nucleotide sequence of coat protein (CP) gene of PVMV (EU719467).  This is the first report of PVMV infection on lisianthus.

Key words:Lisianthus, Pepper veinal mottle virus

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