Journal of Taiwan Agricultural Research

Author:Ting-En Dai* and Chou-Tou Shii

Abstract:

    To investigate the effects of exogenous ABA on the development of scale leaf, four different clones of L. formosanum (F), L. longiflorum (L), oriental hybrid ‘Casa Blanca’ and related distant hybrids (F × CA, L × CA) were in vitro cultured under different ABA, Fluridone and sugar concentration.  Four and eight weeks after culture, it showed that the growth of scale leaves among all genotypes were promoted by ABA but inhibited by Fluridone, ABA can diminish the effects of Fluridone, and Fluridone treatment increases the number of scale leaves but somewhere bleaching in leaf blade.  In applied ABA or Fluridone, L/O hybrids showed intermediate performance as compared with F and L populations.  Four weeks after culture, un supplement both ABA and Fluridone, L/O hybrids had better scale leaf growth than their parents.  Based on the analysis of ABA concentration, it showed that the endogenous of ABA concentration was the highest in CA, lowest in F and L, and L/O hybrids were intermediate, exogenous ABA stimulates endogenous ABA synthesize in the scale, Fluridone inhibits endogenous ABA concentration in the scale, and exogenous ABA can diminished the effects of Fluridone.  The effects of sucrose on the scale leaf morphogenesis showed that higher sucrose concentration inhibits the development of scale leaves.  However, this effect of inhibition was less than exogenous ABA, since scale leaf emergence was still observed in sucrose treatment.  In additionally, high concentration sucrose combined with Fluridone can promote the scale leaf development.  Whatever supplement Fluridone or not in the medium, the effects of high concentration sucrose showed no significant change on endogenous ABA in scale of F, L and CA but increase in L/O hybrids.

Key words:Lily, ABA, Fluridone, Sucrose, Scale leaf

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