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Genetic Diversity Analysis of Bletilla Species Using RAPD Technique
Flower and lip morphological traits of Bletilla formosana collected from various areas of Taiwan and B species from China. Names of tested samples are listed in Table 1.
Flower and lip morphological traits of Bletilla formosana collected from various areas of Taiwan and B species from China. Names of tested samples are listed in Table 1.

Author:Chin-Yi Tsao, Cheng-Zhi Xu, Fu-Shin Chueh, Uei-Chern Chen, and Chi-Ni Hsia*

Abstract:

    Genetic makers of random amplified polymorphic DNA (RAPD) were used to analyze 25 samples containing different species in Bletilla genus and B. formosana collected from different regions of Taiwan.  A total 12 primers with high reproduction was screened from 80 RAPD primers for consecutive experiments.  A total of 165 DNA fragments was detected, among them, 161 markers (97.6%) were polymorphic.  Three major groups of Bletilla genus from China, Japan, and Taiwan were separated according to unwighted pair-group mean arithmetical (UPGMA) analysis in this study.  Five sub-groups of B. fomosana were classified and Bletilla species collected from Lanyu Island was found in the same group of Taiwan.  It assumed that Bletilla formosana (Hayata) Schltr. f. kotoensis (Hayata) T. P. Lin is the same species with B. fomosana.  The geographical barrier of mountains was presumed for constituting the genetic differentiation among ethnic groups of B. formosans in Taiwan.

Key words:RAPD, Bletilla, Genetic diversity, Molecular marker

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Journal of
Taiwan Agricultural Research
Vol. 72, No.3 (2023) ISSN: 2790-086X Vol. 72, No.3 (2023)
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