Journal of Taiwan Agricultural Research

Author:Chi-Ni Hsia*, Jian-Tan Huang, Uei-Chern Chen, Chin-Yi Tsao, Shu-Hui Liang, and Shin-Sheng Tsay

Abstract:

    This study was conducted to investigate effects of concentration, exposure duration of colchicine and treating colchicine with liquid or solid agar medium on in vitro induction of polyploidy from rhizomes of Anoectochilus formosanus.  Ploidy of shoots derived from rhizome explants were analyzed using a flow cytometry.  When the 12-week-old rhizome explants were cultured for two weeks in a solid agar medium or in a liquid medium containing 0, 0.025, 0.125, and 0.625 mM colchicine, the induction rate of polyploidy was the highest (44.8%) for the regenerated shoots in the liquid culture containing 0.625 mM colchicine.  When the 10-, 11-, and 14-week-old rhizome explants were cultured for 3 days on a solid agar medium containing 0, 0.025, 0.25, and 2.5 mM colchicine, the induction rate of polyploidy (42.4%) was the highest in the samples of 10-week-old and 14-week-old rhizomes on the medium containing 2.5 mM colchicine.  Moreover, the 14-week-old rhizomes produced higher number of polyploidy plants than that of 10-week-old rhizomes because the former had a higher survival rate.  When the 10-week-old rhizomes were cultured for 16, 24, 48, and 72 hours in a liquid medium containing 2.5 mM colchicine, the earliest polyploidy shoots showed in the treatment of 16 hour but the highest rate of tetraploid was found in the treatment of 24 hour (45.5%).  This study reveals that the 10-week-old rhizomes of A. formosanus are suitable explants for in vitro production of polyploidy plants as they produced high rate (54.6%) of polyploidy plants after treating for 24 hour in the liquid culture containing 2.5 mM colchicines.

Key words:Anoectochilus formosanus, Micropropagation, Colchicine, Polyploidy, Flow cytometry

Download: PDF Links